Endothelial dysfunction and atherothrombosis
- Principle investigator:
- Gines Escolar
- Contact details:
The main objectives of this research line are:
- Study of the mechanisms involved in the development of bleeding and thrombotic disorders.
- Evaluation of the physiological and pathological implications of circulating and/or intraplatelet tissue factor in atherothrombosis.
- Characterization of endothelial dysfunction as a common factor in the development of complications associated with multiple diseases.
- Evaluation of the participation of serotoninergic and purinergic mechanisms in platelet function. Study of the antithrombotic potential of pharmacological inhibitors.
The most common techniques that will be used are:
- Adhesion studies under flow conditions.
- Flow cytometry (circulating endothelial cells, endothelial progenitors and platelets).
- Electrophoresis, western-blot and immunoprecipitation.
Some of the relevant publications that should be considered are seen below:
- Lopez-Vilchez et al. Redistribution and hemostatic action of recombinant activated factor VII associated with platelets. Am J Pathol. 2011;178:2938-48.
- Hanzu FA et al. Translational evidence of endothelial damage in obese individuals: inflammatory and prothrombotic responses. J Thromb Haemost. 2011;9:1236-45.
- Palomo M, et al. Defibrotide prevents the activation of macrovascular and microvascular endothelia caused by soluble factors released to blood by autologous hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2011;17:497-506.
Follow link for a detailed list:
During the progression of our research, our group has characterized the endothelial dysfunction, developed in different pathologic situations (chronic kidney disease, obesity, hematopoetic stem cell transplantation) associated with cardiovascular and/or thrombotic risks, at different levels: cellular, protein, and epigenetic. An in vitro experimental model of endothelial damage in association with different pathologic conditions associated with the development of atherothrombosis has been also established.
Endothelial dysfunction, inflammation, tissue factor, signalling pathways, adhesion receptors, NFkappaB
The treatment cost of cardiometabolic diseases has important social and family implications. The health concern on this problem fully justifies the investigation of the causes involved in triggering the associated cardiovascular events. A better knowledge of the pathophysiology of cardiovascular diseases and the causes that trigger its progression would help to the implementation or development of new diagnostic tools and therapeutic strategies for prevention and treatment of these complications.
The candidate will be trained in our main techniques, introduced to our research and supervised during the whole period.
Once trained, the candidate should be able to design his/her own research, analyze the results, prepare a manuscript, and defend the results in front of the scientific community.
Team strategic objective in IDIBAPS
It is strategically relevant to focus on:
- Study of the basic mechanisms regulating blood cell function and of the interactions between such cells, with the vascular wall, and with other cells.
- Evaluation of congenital and acquired defects in hemostasis and their pharmacological and/or transfusional correction.
- Laboratory characterization of bleeding and thrombotic phenotypes and genotypes.
- Analysis of the efficacy and safety of new strategies for sparing blood component transfusions.
- Development and evaluation of the efficacy of synthetic platelet substitutes.
- Characterization of animal models of hemostasis, preferentially in mice.