Plataformes

Electronic Microscopy Unit (UB)

Services:

Transmission electron microscopy (TEM)
  • Ultrastructure studies of tissues and cell cultures (on different subtrates)
  • Studies of suspensions (bacteria, virus, flagella, lipid vesicles...)
  • Studies of cellular organelles extraction (reticulum, mitochondria, exosomes...)
  • Molecular localization and colocalization of proteins, glucidic residues,..
  • Molecular localization of DNA or RNA sequences by “in situ” hybridation
  • Cytochemical studies (enzymatic activity, enzymatic digestions..)
  • Correlative techniques (optical, confocal and electron microscope)
  • Preparing of samples for TEM-based microanalysis
  • Studies of viruses, organules and proteins by negative staining.
Scanning electron microscopy (SEM)
  • Microstructural analysis of surface cell cultures on different substrates (glass, thermanox, aclar,..)
  • Surface characterization of tissues or organs
  • Studies of cellular suspensions(bacteira...)
  • Studies of surfaces: bony, teeth, cornea, skin,...
  • Characterization of biofilms and their formation
  • Morphological studies of small organisms and micro-organisms
  • Surface molecular localization
  • Characterization of biomaterials, implants...
  • Study, analysis and assessment of the images
Examples of applications
  • Biomedical characterization of any kind of cell culture or tissues by SEM or TEM
  • Studies of markers incorporation (colloidal gold, quantum-dots..) inside the tissues or cell culture (ex. Inhalation of nanogold and their localisation by light and transmissionn electron microscopy)
  • Sperm cell study by TEM and SEM for diagnosis
  • Studies of biofilms on water filters, tracheal tubes, historical monuments.
  • Characterization of protein aggregates for example in Alzheimer’s disease
  • Biopsies in pathology
  • Calcium microanalysis detection
Sample preparation types:

Cryomethods preparation (cryofixation)

  • High pressure cryofixation, cryosubstitution, cryo embedding in epoxy resins ( for ultrastructural studies)
  • High pressure cryofixation, cryosubstitution, cryo embedding in acrylic resins at low temperatures (molecular location studies: immunolocalization, "in situ" hybridization, etc.)
  • High pressure cryofixation, cryosubstitution, critical point process (3-D studies using scanning electron microscopy)

Standard preparation method (chemical fixation)

  • Chemical fixation, dehydration and embedding in epoxy resins for ultrastructural studies
  • Ultramicrotomy at room temperature
  • Negative staining (viruses, flagella, proteins, etc.)
  • Cytochemical techniques (detection of enzymes, glycoproteins, sugars, etc.)
  • Chemical fixation, dehydration, critical point (scanning electron microscopy)

Mixed preparation: combination of standard methods and cryomethods

  • Chemical fixation, cryoprotection, cryosubstitution and embedding in epoxy resins (ultrastructural study)
  • Chemical fixation, cryofixation and embedding in epoxy resins (ultrastructural study)
  • Chemical fixation, cryoprotection, cryosubstitution and embedding at low temperatures (molecular localization studies: immunolocalization, "in situ" hybridization, etc.)
  • Chemical fixation, cryoprotection, cryofixation and cryoultramicrotomy (molecular location studies: immunolocalization, "in situ" hybridization, etc.)
  • Chemical fixation, cryofixation, cryosubstitution, critical point process (for scanning electron microscopy)

Molecular localization

  • Proteins, sugar residues,...
  • Markers: nanogold, silver enhancement, colloidal gold, quantum-dots, lectins.

Correlative techniques

  • To observe the “same” cell (for example a dynamic process) by light microscopy (at low resolution) and by transmission electron microscopy (higher resolution) using cryofixtion or chemical fixation.

Equipment:

  • Jeol EM 1010 transmission electronic microscope
  • Zeiss DSM 940A scanning electron microscope
  • EM PACT cryofixation system (Leica)
  • Cryosubstitution systems
    • AFS (Leica)
    • AFS2 (Leica)
  • Ultramicrotomes
    • Ultracut (Reichert-Jung)
    • Ultracut E (Reichert-Jung)
    • Ultracut UCT (Leica) + FCS Cryoultramicrotomy Unit (Leica)
  • VGMicrotech CPD 7501 critical point system
  • Fisons Instrument FC510 Sputtering System
  • Optical microscopes
    • Leica DMLS
    • Olympus CX41 RF + Olympus DP 70 digital camera

Location

Location

UB Faculty of Medicine
North Wing, 6th floor

Scientific coordinator(s):

Dr Núria Cortadellas
Unit Head
nuriac(ELIMINAR)@sct.ub.es
Tel. 93 403 44 07 - 93 403 98 59

Staff

  • Ms Almudena Garcia
    Ultramicrotomy Section Manager
  • Ms Ana Ribera
    Laboratory technician
  • Ms Eva Fernández
    IDIBAPS technician – research support contract