Plataformes

Biobank

Introduction

The Biobank is a centralised institutional support core facility for coordinating the collection, processing, storage and transfer of human biological samples to promote biomedical research of the highest standard.

With the aim of optimizing the human resources and equipment in the Biobank, this facility offers a wide range of services for the processing and analysis of the samples gathered by the research groups.

All the procedures performed at the Biobank are carried out under ISO9001 certification.

When it was first set up, the Biobank had a dual mission:

  • To provide the scientific community with standard, well characterised biological samples with high added value, in order to promote, foster and develop biomedical research in accordance with current legislation
  • To standardise all the Institute's research sample collections (in accordance with article 67 of Law 14/2007 and the Real Decreto RD1716/2011 related to Biomedical Research with human samples)

The bank’s catalogue of samples is formed by the samples collected by three large banks, all of them with an extensive experience in research with human samples and which are available for scientific purposes for the consolidation of personalized medicine:

  1. Neurological Tissue bank
    A nervous tissue repository (brain and spinal cord) created from donations from cadavers with or without neurological conditions, helping to facilitate research into neurological illnesses in order to improve diagnoses and enhance patient assistance.
  2. Tumour bank and Anatomical Pathologies Collections
    A Repository of tumoural and non-tumoural tissue samples from patients affected by a neoplasia, as well as tissue samples from other diseases, all of them being surplus to diagnostic requirements from the Department of Anatomical Pathology in the Hospital Clínic. This material is essential to providing current large-scale tissue research projects with the samples they require.
  3. Blood and Fluid bank
    This repository contains samples of metabolic, inflammatory bowel, oncologic, digestive, psychiatric and maternal and foetal diseases, among others. It houses a large range of samples of scientific interest, primarily DNA, serum and plasma, which are provided by doctors’ surgeries and laboratories where blood and fluid are taken for lab tests and blood and fluid donation.

Services:

The Biobank of Hospital Clínic – IDIBAPS offers a wide variety of human biological samples along with services. The scientific community may make full use of the bank’s equipment and professional staff to achieve their most ambitious goals. The work of the biobank goes beyond simply providing samples; they offer support to researchers during the various stages of projects requiring the use of biological samples.

  • HUMAN BIOLOGICAL SAMPLE PROVIDING
    • Nerve tissue from cadaveric donors affected by neurologic diseases
    • Frozen samples representing the most common neoplasias and paraffin-embedded tissues from a broad spectrum of neoplastic and non-neoplastic diseases.
    • DNA, serum, plasma and PBMCs from different diseases as well as from control donors
  • TECHNICAL AND SCIENTIFIC ADVICE
    • Creation and management of new collections
    • Methodology (technical guidance)
    • Experimental design with human and animal tissue samples
    • Design and interpretation of immunohistochemical studies
    • Quality management programmes
    • Harmonization of quality procedures for the handling of DNA samples
    • Technical assessment for the manipulation of the Biobank equipment (laser microdissector, multiobserver microscope with a software for morphometric analysis)
  • PRIVATE COLLECTIONS MANAGEMENT
    • Collection management of multicentre studies
    • Storage of pre-existing collections
  • HISTOLOGICAL TECHNIQUES
    • Processing of frozen and paraffin embedded tissues (macroscopy, cryopreservation, paraffin blocks, histological sections)
    • Lasser microdissection
    • Tissue microarrays construction (TMA)
    • Histological staining
    • Definitive neuropathological diagnosis of neurological diseases
    • Slide scan and morphometric analysis
  • CELLULAR TECHNIQUES
    • Purification and cryopreservation of mononuclear cells
    • Immortalization of B lymphocytes
    • Erythrocyte isolation from whole blood
    • Cell counting
  • MOLECULAR TECHNIQUES
    • DNA extraction from peripheral blood
    • DNA/RNA/protein extraction from tissue (frozen and paraffin embedded)
    • WGA
    • Serum and plasma isolation
    • Immunohistochemistry

Equipment:

  • -20ºC, -80ºC and -150ºC freezers with safety systems
  • Vapour phase liquid Nitrogen tanks

Neurological tissue bank

  • Cubicles for obtaining the material in sterile conditions
  • Instruments for preparing samples for the microscope
  • Filing cabinets for collecting paraffined samples
  • Gas filtration cabinet
  • Automated immunostaining appliance (DAKO autostainer plus)
  • Combination refrigerator and -40ºC freezer for preserving fungibles
  • Autoclave for sterilising surgical material
  • Microtomes and cryostat (Leica Microsystems)

Tumour bank

  • Equipment for preparing tissue microarrays (TMAs): TMA Master (3Dhistech)
    (UB Faculty of Medicine / FEDER)
  • LaserTissue microdissection microscope with laser LMD7000 (Leica Microsystems)
  • Microtomes and cryostat (Leica Microsystems)
  • Tissue processor (Leica Microsystems)
  • Bond Max III for automated immunohistochemistry (Leica Microsystems)
  • Digital slide scanner Pannoramic Desk (3Dhistech)
  • Multiobserver microscope BX43 with a 12Mpx photographic camera and the morphometric analysis software CellSens Dimension (Olympus)
  • Automated molecular extraction by the QIAcube (Qiagen)

Blood and Fluid Biobank

  • Programmable freezer with a controlled freezing ramp
  • “Chemagic MSM1®” automated device for obtaining DNA from large volumes of blood
  • "Tecan EVO150®” automated station for the aliquoting of samples
  • “CoulterZ1®” cells and particles counter
  • TC Automated Cell Counter (Bio Rad)
  • Cabinets for isolating cells in sterile conditions
  • Cell immortalisation platform
  • Computer systems for managing samples and related data
  • ABF500 Automated Blood Fractionation system (RTS)
  • Automated molecular extraction by the QIAcube (Qiagen)

Location

Location

Neurological Tissue bank
Faculty of Medicine UB.
South wing, floor 0

Tumour bank
Centre Esther Koplowitz
c/ Rosselló, 149-153, planta -1

Servei d’Anatomia Patològica (Hospital Clínic)
c/ Villarroel, 170. Escala 3, planta 5

Blood and Fluid bank
Centre Esther Koplowitz
c/ Rosselló, 149-153, planta -1

biobanc(ELIMINAR)@idibaps.org

Services request

Scientific coordinator

Anna Bosch

Anna Bosch Comas, PhD (IDIBAPS)
anna.bosch(ELIMINAR)@idibaps.org

Staff

Biobank
Neurological tissue bank
  • Ellen Gelpí, MD, PhD (IDIBAPS)
    ellen.gelpi(ELIMINAR)@idibaps.org
  • Sara Charif (IDIBAPS)
    Laboratory technician
  • Verónica Santiago (IDIBAPS) 
    Laboratory technician
  • Abel Muñoz (IDIBAPS)
    Autopsy Assistant
  • Leire Etxarri (IDIBAPS)
    Autopsy Assistant
Tumour bank
  • Miriam Cuatrecasas, MD, PhD (Hospital Clínic)
    mcuatrec(ELIMINAR)@clinic.ub.es
  • Laura Gelabert (IDIBAPS)
    Laboratory technician
  • Mónica Marín (Hospital Clínic)
    Laboratory technician
  • Raquel Bermudo Gascón, PhD (IDIBAPS)
    Bank Manager
  • Ángela Martín (Fundació Clínic) 
    Informed Consent Manager
    angela.martin(ELIMINAR)@idibaps.org
  • Anatomic Pathology residents (Hospital Clìnic - IDIBAPS)
    Histopathological evaluation of samples
Blood and Fluid Bank