Plataformes

Cytometry and Cell Sorting Facility

Introduction

Flow cytometry is a technique for multiparametric analisys of particles, such as cells , by suspending them in a stream of fluid and passing them through a LASER. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second. Each suspended particle passing through the beam scatters the LASER, and fluorescent chemicals found in the particle or attached to the particle may be excited into emitting light at a longer wavelength than the light source.

Fluorescence-activated cell sorting is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest.

These technology has applications in molecular biology, pathology, immunology, plant biology and marine biology. It has broad application in medicine (especially in transplantation, hematology, tumor immunology and chemotherapy, prenatal diagnosis, genetics…).

The Facility is subdivided into three rooms: FACS analysis and high resolution images in flow room , data analysis room and cell sorting room.

Services:

  • Analysis of population of interest.
  • Apoptosi.
  • Cell cycle, Proliferation.
  • Functional Analysis.
  • Stem Cells.
  • Viability.
  • Analysis of Microorganism.
  • Multiparameter quantitative trials on soluble molecules and phosphoproteins (CBA and CBA Flex Set), Cell signaling.
  • Cell sorting.
  • Microparticles.
  • High resolution microscopy in flow (Cell Signaling and molecular translocation, Internalization, Co-localization, Cell-cell Interaction, Morphology Analysis, Cell Death and Autophagy, Cell Cycle and Mitosi, DNA damage and repair).
  • User training.

For equipment booking without staff assistance , please sign up: www.quartzy.com. Once registered, login and  join group CORE FACILITIES-FLOW CYTOMETRY www.quartzy.com/groups.

For equipment booking with staff assistance, please send request to citometria(ELIMINAR)@idibaps.org.

Equipment:

  • BD FACSCalibur: 2 LASER, Up to 4 fluorescence.
  • BD FACSCantol : 2 LASER, Up to 6 fluorescence.
  • BD FACSCantoII: 3 LASER, Up to 8 fluorescence.
  • BD LSRFortessa SORP + HTS: 4 LASER, Up to 16 fluorescence.
  • BD LSRFortessa SORP: 5 LASER, Up to 18 fluorescence.
  • AMNIS ISX : 2 digital cameras, Up to 12 fluorescence channels.
  • BD FACSAriaII: 3 LASER, Up to 9 fluorescence.
  • BD FACSAria SORP: 5 LASER, Up to 18 fluorescence.
  • Magnetic cell sorter autoMACSPro.
  • Data analysis: DIVA v8.0, CellQuestPro v6.0.3, ModFit LT v3.2, FlowJo v10, FCAPArray v1.0.1.

Location

Location

C/ Roselló 149-153, -1 Floor.
Cytomics Core Facility CEK
citometria(ELIMINAR)@idibaps.org

Services request

Scientific coordinator and Head of Facility:

Isabel Crespo

Isabel Crespo, PhD. (IDIBAPS)
isabel.crespo(ELIMINAR)@idibaps.org

Staff

  • Cristina López (IDIBAPS)
    Specialist Technician
  • Cristina Xufré (IDIBAPS)
    Specialist Technician