IDIBAPS, Institut d'Investigacions Biomèdiques August Pi i Sunyer

Services:

Flow cytometry measures and analyzes the characteristics of single particles, normally cells, as they move in a stream and are passed through a laser. Thousands of cells can be analyzed by a flow cytometer in a single second. It is possible to mark the particles with one or more fluorescent substances that then makes it possible to measure physical characteristics such as cell size, shape, internal complexity and any cell component or function that can be detected by a fluorescent compound.

The applications of flow cytometry are numerous, and this has led to the widespread use of these instruments in the biological and medical fields.

The facility is subdivided into four groups: FACS analysis, data analysis, cell sorting and high resolution images of each cell directly in flow.

1. Analysis of population of interest (Up to 18 colors)
   • FACSCalibur (Up to 4 colors)
   • FACSCantoI (Up to 6 colors)
   • FACSCantoII (Up to 8 colors)
   • LSRFortessa SORP HTS 4 LASER (Up to 16 colors)
   • LSRFortessa SORP 5 LASER (Up to 18 colors)
2. Apoptosi
   • FACSCalibur
   • FACSCantoI
   • FACSCantoII
   • LSRFortessa SORP HTS 4 LASER
   • LSRFortessa SORP 5 LASER
3. Cell cycles
   • FACSCalibur
   • FACSCantoI
   • FACSCantoII
   • LSRFortessa SORP HTS 4 LASER
   • LSRFortessa SORP 5 LASER
4. Functional Analysis
   • FACSCalibur
   • FACSCantoI
   • FACSCantoII
   • LSRFortessa SORP HTS 4 LASER
   • LSRFortessa SORP 5 LASER
5. Stem Cells
   • LSRFortessa SORP 5 LASER
6. Viability
   • FACSCalibur
   • FACSCantoI
   • FACSCantoII
   • LSRFortessa SORP HTS 4 LASER
   • LSRFortessa SORP 5 LASER
7. Multiparameter quantitative trials on soluble molecules and phosphoproteins (CBA and CBA Flex Set)
   • FACSCalibur
   • FACSCantoI
   • FACSCantoII
   • LSRFortessa SORP HTS 4 LASER
   • LSRFortessa SORP 5 LASER
8. Cell sorting
   • autoMACS (magnetic sorter)
   • FACSAria II (Up to 9 colors)
   • FACSAria SORP 5 LASER (Up to 18 colors)
9. High resolution microscopy in flow (Cell Signaling and molecular translocation, Internalization, Co-localization, Cell-cell Interaction, Morphology Analysis, Cell Death and Autophagy, Cell Cycle and Mitosi, DNA damage and repair)
   • ImageStreamX : 2 cameras, 4 LASER (AMNIS Corporation)

Equipment:

• FACSCalibur cytofluorimeter (IDIBAPS)
• FACSCantoll cytofluorimeter (IDIBAPS)
• Vantage SE FACS cell sorter (IDIBAPS)
• FACSAriaII cell sorter (IDIBAPS)