IDIBAPS, Institut d'Investigacions Biomèdiques August Pi i Sunyer

Services:

Light microscopy:

• Confocal laser microscopy
• Two-photon excitation microscope (multiphoton)
• Wide-field microscopy

Light microscopy applications:

• Fluorescent, reflection and transmitted light, multi-channel and multidimensional (xyztλ) images. Mosaic and multi-position images
• Live cell microscopy.
• Molecular proximity analysis: FRET (Fluorescence resonance energy transfer)
• Molecular dynamics analysis by photobleaching and photolabelling techniques: 
  • FRAP (Fluorescence recovery after photo-bleaching);
  • Inversed FRAP,
  • FLIP (Fluorescence loss in photo-bleaching);
  • Photo-activation, photo-conversion.
• Intravital Microscopy (live animal):
  • Confocal, multi-photon;
  • Resonant scan (high speed).
• Two photon excitation microscopy (Multi-photon):
  • Fluorescence;
  • Second Harmonic Generation.

Cell culture and micromanipulation:

• Maintenance of cell lines for microscopy experiments.
• Microinjection.
• Molecular and cellular dyes (transfection, staining)

Image processing and analysis:

• Technical assistance in image processing and analysis (3D reconstruction, quantification, co-localization, FRET analysis, molecular dynamics analysis, particle tracking)
• Development of custom instructions macros.
• Training days of image processing and analysis.

User support and innovation:

• Assistance in planning, preparation, performance and analysis of light microscopy experiments.
• Training in the use of equipment.
• Development of new methodologies.

Equipment:

• Multiphoton and high-velocity spectral confocal microscope (Leica TCS-SP5)
• Spectral confocal microscope (Leica TCS-SL)
• Confocal microscope (Leica TCS-NT)
• Microinjector (Leica AS TP)
• Wide-field fluorescence microscope (Leica DMI6000 B) (Faculty of Medicine)
• General laboratory
• Culture room
• Imaging analysis workstations